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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
10/11/2023 |
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Actualizado : |
10/11/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BORGES, M.A.; CURCIO, B.R.; GASTAL, G.D.A.; GHENO, L.; JUNIOR, A.S.V.; CORCINI, C.D.; NOGUEIRA, C.E.W.; AGUIAR, F.L.N.; GASTAL, E.L. |
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Afiliación : |
MORGANA A. BORGES, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; BRUNA R. CURCIO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIZA GHENO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; ANTONIO S. VARELA JUNIOR, Department of Comparative Animal Reproduction, Institute of Biological Sciences, Federal University of Rio Grande, RS, Rio Grande, Brazil; CARINE D. CORCINI, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; CARLOS E.W. NOGUEIRA, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; FRANCISCO L.N. AGUIAR, Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraiba, PB, Sousa, Brazil; EDUARDO L. GASTAL, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States. |
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Título : |
Ethanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Reproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814 |
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ISSN : |
1642-431X |
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DOI : |
10.1016/j.repbio.2023.100814 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. -- |
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Contenido : |
ABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn MenosABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more ... Presentar Todo |
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Palabras claves : |
Fixative solution; Mare; Ovarian tissue quality; Preantral follicle morphometry. |
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Asunto categoría : |
L50 Fisiología y bioquímica animal |
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Marc : |
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001 1064365
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024 7 $a10.1016/j.repbio.2023.100814$2DOI
100 1 $aBORGES, M.A.
245 $aEthanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. --
520 $aABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
653 $aFixative solution
653 $aMare
653 $aOvarian tissue quality
653 $aPreantral follicle morphometry
700 1 $aCURCIO, B.R.
700 1 $aGASTAL, G.D.A.
700 1 $aGHENO, L.
700 1 $aJUNIOR, A.S.V.
700 1 $aCORCINI, C.D.
700 1 $aNOGUEIRA, C.E.W.
700 1 $aAGUIAR, F.L.N.
700 1 $aGASTAL, E.L.
773 $tReproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814
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| 103. |  | CARRASCO-LETELIER, L.; VÁZQUEZ, D.; RESQUÍN, F.; RACHID, C.; D´OTTONE, F.; TERRA, J.A.; SCOZ, R.; VILARO, F.; SALDIAS, R.; OTEGUI, O.; SOUTO, G. Evaluando la sostenibilidad energética de las agroenergías Revista INIA Uruguay, 2011, no. 27, p. 48-51 (Revista INIA; 27)| Tipo: Artículos en Revistas Agropecuarias |
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| 104. | | VÁZQUEZ, D.; CARRASCO-LETELIER, L.; D´OTTONE, F.; RESQUIN, J.; SCOZ, R.; VILARO, F.; RODRIGUEZ, G.; VICENTE, E.; TERRA, J. Evaluating the sustainability of potential agro-industrial chains (sweet sorghum, grain sorghum, sweet potato and forestry) for agroenergy production. In: USDA-INIA Workshop of ECPA Project "Evaluating the Sustainability of Potential Agro-Industrial Chains for Agroenergy Production", 2013.| Tipo: Trabajos en Congresos/Conferencias |
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| 105. | | SPATARI, S.; ADLER, P.R.; CARRASCO-LETELIER, L.; D´OTTONE, F.; VÁZQUEZ, D.; RODRÍGUEZ, G.; VICENTE, E.; VILARO, F. Evaluation of low carbon biofuels for domestic energy supply in Uruguay. In: CANADIAN CHEMICAL ENGINEERING CONFERENCE, 62., INTERNATIONAL SYMPOSIUM ON BIOMASS AND BIOENERGY, 2012, Vancouver, BC, CA. Building the bio-economy: 583. [s.l.]: O`Zone Software, 2012.| Biblioteca(s): INIA La Estanzuela. |
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| 108. | | ALTESOR, P.; GARCÍA, A.; FONT, E.; RODRÍGUEZ-HARALAMBIDES, A.; VILARO, F.; OESTERHELD, M.; SOLER, R.; GONZÁLEZ, A. Glycoalkaloids of Wild and Cultivated Solanum: Effects on Specialist and Generalist Insect Herbivores. Journal of Chemical Ecology, 2014, v.40, no.6, p.599-608. Article history: Received: 25 March 2014 /Revised: 5 May 2014 /Accepted: 9 May 2014 / Published online: 27 May 2014.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
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| 109. | | Bernal, R.Buenahora, J.Paullier, J.Maeso, D.Arboleya, J.Campelo, E.Falero, M.Núñez, S.Leoni, C.Vicente, E.Vilaró, F.Spina, W.Manzzioni, A.Rodríguez, G.Pereira, G.González, M.Giménez, G. Hacia una agricultura responsable y sustentable Salto (Uruguay): INIA, 2009. 18 p (INIA Serie Actividades de Difusión ; 579)| Biblioteca(s): INIA Las Brujas. |
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| 110. | | Taller, 2009, Salto, Uruguay - Bernal, R.; Buenahora, J.; Paullier, J.; Maeso, D.; Arboleya, J.; Campelo, E.; Falero, M.; Núñez, S.; Leoni, C.; Vicente, E.; Vilaró, F.; Spina, W.; Manzzioni, A.; Rodríguez, G.; Pereira, G.; González, M.; Giménez, G. Hacia una agricultura responsable y sustentable Salto (Uruguay): INIA, 2009. 18 p (INIA Serie Actividades de Difusión ; 579) INIA Salto Grande| Biblioteca(s): INIA Tacuarembó. |
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| 118. | | NARANCIO, R.; ZORRILLA, P.; ROBELLO, C.; GONZÁLEZ-ARCOS, M.; VILARÓ, F.; PRITSCH, C.; DALLA RIZZA, M. Insights on gene expression response of a characterized resistant genotype of Solanum commersonii Dun. against Ralstonia solanacearum. European Journal of Plant Pathology, 2013, v.136, no.4, p.823-835. Article history: Accepted: 27 March 2013 / Published online: 28 April 2013.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 1 |
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| 119. | | FERREIRA, V.; PIANZZOLA, M.J.; VILARÓ, F.; GALVÁN, G.; TONDO, M.L.; RODRÍGUEZ, M.V.; ORELLANO, E.G.; VALSS, M.; SIRI, M.I. Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. Frontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS. Article history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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